APC is epigenetically down regulated in advance cases of gallbladder cancer
- Resource Type
- Authors
- Manisha Kakkar; Braj Raj Shrivastav; Vishwajit Jalaj; Shushruta Bhunia; Pramod Kumar Tiwari; Satish S. Poojary; Mustafa A. Barbhuiya; Sanjeev Gupta; Dinesh Tekcham Singh
- Source
- Molecular Cytogenetics
- Subject
- Biochemistry, medical
congenital, hereditary, and neonatal diseases and abnormalities
medicine.medical_specialty
Biochemistry (medical)
Bisulfite sequencing
Cytogenetics
nutritional and metabolic diseases
Biology
Bioinformatics
medicine.disease
Molecular medicine
Biochemistry
Human genetics
genomic DNA
Exon
Poster Presentation
medicine
Cancer research
Genetics
Immunohistochemistry
Molecular Medicine
Genetics(clinical)
Gallbladder cancer
Molecular Biology
Genetics (clinical)
- Language
- English
- ISSN
- 1755-8166
Methods We carried out methylation specific PCR of the modified genomic DNA from GBC and GSD tissues, followed by semi-quantitative and quantitative PCRs (qPCR). We compared the transcript levels of the two exons of APC in GBC and GSD as compared to their adjacent control tissues. We also performed Immunohistochemistry (IHC) on tissue micro array (TMA) of GBC and GSD. The scoring of different tissue cores was done by the expert pathologist and student’s t-test was performed to check the significance.