Effects of silodosin, a selective alpha-1A adrenoceptor antagonist, on erectile function in a rat model of partial bladder outlet obstruction
- Resource Type
- Authors
- Nur Bayatli; Alp Özgür Akdemir; Serap Gur; Ecem Kaya; Tugce Bastaskın; Didem Yilmaz; Isil Ozakca
- Source
- Neurourology and Urodynamics. 36:597-603
- Subject
- Mean arterial pressure
medicine.medical_specialty
business.industry
Urology
Urinary bladder neck obstruction
030232 urology & nephrology
Silodosin
urologic and male genital diseases
medicine.disease
Masson's trichrome stain
03 medical and health sciences
Bladder outlet obstruction
0302 clinical medicine
Urethra
medicine.anatomical_structure
Erectile dysfunction
Lower urinary tract symptoms
030220 oncology & carcinogenesis
medicine
Neurology (clinical)
business
medicine.drug
- Language
- ISSN
- 0733-2467
Aim We investigated the effects of silodosin (selective α1A-adrenoceptor antagonist) on erectile dysfunction (ED) in a rat model of bladder outlet obstruction. Methods Adult male Sprague–Dawley rats (n = 32) were divided into four groups: (i) sham-operated control; (ii) silodosin-treated (sham) control (0.1 mg/kg/day); (iii) partial bladder outlet obstruction (PBOO); and (iv) silodosin-treated with PBOO. PBOO was induced by ligation of the urethra for 6 weeks. In vivo, erectile responses were monitored by evaluating ratios of intracavernosal pressure (ICP)/mean arterial pressure (MAP). Organ-bath studies were performed on corpus cavernosum (CC) strips. Penises were assessed at baseline for protein expression of neuronal nitric oxide synthase (nNOS) and Rho-associated protein kinase (ROCK2) by Western blot. Immunohistochemistry and Masson trichrome staining were performed for analysis of nNOS protein levels and tissue alterations. Results The ratio of ICP/MAP was significantly decreased in obstructed rats (0.26 ± 0.043, P