Virological and serological characterization of critically ill patients with COVID-19 in the UK: Interactions of viral load, antibody status and B.1.1.7 variant infection
- Resource Type
- Authors
- Ratcliff, J; Nguyen, D; Fish, M; Rynne, J; Jennings, A; Williams, S; Al-Beidh, F; Bonsall, D; Evans, A; Golubchik, T; Gordon, AC; Lamikanra, A; Tsang, P; Ciccone, NA; Leuscher, U; Slack, W; Laing, E; Mouncey, PR; Ziyenge, S; Oliveira, M; Ploeg, R; Rowan, KM; Shankar-Hari, M; Roberts, DJ; Menon, DK; Estcourt, L; Simmonds, P; Harvala, H
- Source
- The Journal of Infectious Diseases
- Subject
- Male
Critical Illness
Antibodies, Viral
Microbiology
Polymerase Chain Reaction
Major Article
Humans
Serologic Tests
clade B.1.1.7
11 Medical and Health Sciences
COVID-19 Serotherapy
Aged
SARS-CoV-2
SARS-CoV-2 COVID-19
Immunization, Passive
COVID-19
06 Biological Sciences
Middle Aged
Viral Load
randomized clinical trial
Antibodies, Neutralizing
United Kingdom
REMAP-CAP Immunoglobulin Domain UK Investigators
Coronavirus
AcademicSubjects/MED00290
Immunoglobulin G
convalescent plasma
Spike Glycoprotein, Coronavirus
RNA, Viral
ELISA
Female
variant of concern
- Language
- English
- ISSN
- 1537-6613
0022-1899
Background Convalescent plasma containing neutralising antibody to SARS-CoV-2 is under investigation for COVID-19 treatment. We report diverse virological characteristics of UK intensive care patients enrolled in the Immunoglobulin Domain of the REMAP-CAP randomised controlled trial that potentially influence treatment outcomes. Methods SARS-CoV-2 RNA in nasopharyngeal swabs collected pre-treatment was quantified by PCR. Antibody status was determined by spike-protein ELISA. B.1.1.7 was differentiated from other SARS-CoV-2 strains using allele-specific probes or restriction site polymorphism (SfcI) targeting D1118H. Results Of 1274 subjects, 90% were PCR-positive with viral loads 118-1.7x10 11 IU/ml. Median viral loads were 40-fold higher in those seronegative for IgG antibodies (n=354; 28%) compared to seropositives (n=939; 72%). Frequencies of B.1.1.7 increased from