Cross-linked enzyme crystals of fructose diphosphate aldolase: Development as a biocatalyst for synthesis
- Resource Type
- Authors
- Susan B. Sobolov; Anita Bartoszko-Malik; Thomas R. Oeschger; Michelle M. Montelbano
- Source
- Tetrahedron Letters. 35:7751-7754
- Subject
- Ammonium sulfate
biology
education
Organic Chemistry
Aldolase A
Fructose
Biochemistry
humanities
Catalysis
Solvent
chemistry.chemical_compound
chemistry
Biocatalysis
Drug Discovery
biology.protein
Organic chemistry
Reactivity (chemistry)
Glutaraldehyde
- Language
- ISSN
- 0040-4039
Crystals of rabbit muscle aldolase were grown from 45% saturated ammonium sulfate solutions and cross-linked with glutaraldehyde to produce a biocatalyst that has stability for months, can be reisolated and reused, and has excellent solvent stability. This catalyst showed excellent reactivity with non-natural substrates.