Intranasal immunization with a recombinant outer membrane protein H based Haemorrhagic septicemia vaccine in dairy calves
- Resource Type
- Authors
- Ratchanee Uthi; Korkiat Muangthai; Pallop Tankaew; Takuo Sawada; Thanya Varinrak; Suvichai Rojanasthien; Nattawooti Sthitmatee
- Source
- The Journal of Veterinary Medical Science
- Subject
- 0301 basic medicine
Serotype
Pasteurella multocida
040301 veterinary sciences
Cattle Diseases
Enzyme-Linked Immunosorbent Assay
law.invention
Microbiology
0403 veterinary science
03 medical and health sciences
Adjuvants, Immunologic
Antigen
law
CpG-ODN 2007
intranasal vaccine
Animals
Medicine
Hemorrhagic Septicemia
Administration, Intranasal
Full Paper
General Veterinary
biology
business.industry
Bacteriology
04 agricultural and veterinary sciences
Thailand
biology.organism_classification
Antibodies, Bacterial
haemorrhagic septicemia
Virology
Recombinant Proteins
Immunoglobulin A
Vaccination
030104 developmental biology
Immunization
Immunoglobulin G
Bacterial Vaccines
Recombinant DNA
biology.protein
Cattle
Female
recombinant outer membrane protein
Nasal administration
Antibody
business
Bacterial Outer Membrane Proteins
- Language
- ISSN
- 1347-7439
0916-7250
Haemorrhagic septicemia (HS) is a contagious disease in cattle with high morbidity and mortality rates. HS vaccine in Thailand is an oil-adjuvant formulation, and is difficult to administer. The present study aimed to formulate and evaluate the protection in dairy calves conferred by immunization with an in-house intranasal HS vaccine. The intranasal vaccine was formulated in a total volume of 500 µl containing either 50 or 100 µg of the recombinant outer membrane protein H (rOmpH) of Pasteurella multocida strain M-1404 (serovar B:2), and 10 µg of Cytosine-phosphate-guanosine oligodeoxynucleotides (CpG-ODN) as a mucosal adjuvant. Intranasal immunizations were conducted three times at three-week intervals. The antibodies post-immunization were detected by indirect ELISA and demonstrated efficient in vitro activity in suppressing a P. multocida strain from the complement-mediated killing assay. An intranasal vaccine induced both the serum IgG and secretory IgA levels that were significantly higher than the level conferred by the parenteral vaccine (P