Plasma cell-free DNA levels are elevated in acute Puumala hantavirus infection
- Resource Type
- Authors
- Jukka Mustonen; Jaana Syrjänen; Juulia Jylhävä; Mikko Hurme; Sonja Leppänen; Antti Vaheri; Taru Kuparinen; Tuula K. Outinen; Satu Mäkelä; Ilkka Pörsti
- Source
- PLoS ONE, Vol 7, Iss 2, p e31455 (2012)
PLoS ONE
- Subject
- Viral Diseases
Apoptosis
Urine
Plasma cell
Puumala virus
Severity of Illness Index
COLORECTAL-CANCER
chemistry.chemical_compound
Leukocyte Count
INDUCED NEPHROPATHIA-EPIDEMICA
Zoonoses
Blood plasma
RADIOLOGICAL FINDINGS
Nephropathia epidemica
Pathology
PROGNOSTIC MARKER
0303 health sciences
Sisätaudit - Internal Medicine
Multidisciplinary
biology
C-REACTIVE PROTEIN
3. Good health
RENAL SYNDROME
medicine.anatomical_structure
Infectious Diseases
Nephrology
Acute Disease
Medicine
CLINICAL CORRELATIONS
Research Article
medicine.medical_specialty
Hantavirus Infections
Science
education
VIRUS-INFECTION
Excretion
03 medical and health sciences
Diagnostic Medicine
Internal medicine
medicine
Humans
030304 developmental biology
Creatinine
030306 microbiology
business.industry
Platelet Count
C-reactive protein
VERO E6 CELLS
DNA
Length of Stay
medicine.disease
biology.organism_classification
Endocrinology
HEMORRHAGIC-FEVER
chemistry
3121 General medicine, internal medicine and other clinical medicine
Immunology
biology.protein
Clinical Immunology
business
General Pathology
- Language
- English
- ISSN
- 1932-6203
Introduction Puumala hantavirus (PUUV) causes a hemorrhagic fever with renal syndrome called nephropathia epidemica (NE). The aim of the present study was to evaluate plasma cell-free DNA (cf-DNA) levels and urinary cf-DNA excretion in acute NE as well as their associations with the severity of the disease. Methods Total plasma cf-DNA was quantified directly in plasma of 61 patients and urine of 20 patients with acute NE. We also carried out a qualitative high-sensitivity lab-on-a-chip DNA assay in 20 patients to elucidate the appearance of cf-DNA in plasma and urine. Results The maximum plasma cf-DNA values taken during acute NE were significantly higher than the control values taken after the hospitalization period (median 1.33 µg/ml, range 0.94–3.29 µg/ml vs. median 0.77 µg/ml, range 0.55–0.99 µg/ml, P