Dynamic and unpredictable changes in mutant allele fractions of BRAF and NRAS during visceral progression of cutaneous malignant melanoma
- Resource Type
- Authors
- Tamás Barbai; József Tímár; Erzsébet Rásó; Viktória Doma; Sarolta Kárpáti
- Source
- BMC Cancer
BMC Cancer, Vol 19, Iss 1, Pp 1-9 (2019)
- Subject
- Neuroblastoma RAS viral oncogene homolog
Oncology
Cancer Research
medicine.medical_specialty
business.industry
Melanoma
Mutant allele
MEDLINE
Mutant allele fraction
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
medicine.disease
lcsh:RC254-282
BRAF
Metastasis
Text mining
Surgical oncology
Internal medicine
Genetics
medicine
business
neoplasms
Research Article
- Language
- ISSN
- 1471-2407
Background Data indicate that primary cutaneous melanomas are characterized by clonal heterogeneity associated with oncogenic drivers. Less data are available on the clonal changes occurring during melanoma progression. We therefore wished to analyse these changes in skin melanomas in common sites of visceral metastases as compared to the primary tumor. Methods An autopsy cohort of 50 patients with BRAF- and NRAS-mutant cutaneous metastatic melanomas including 139 visceral metastases was analysed for mutant allele fractions (MAF), determined by pyrosequencing and corrected for tumor/normal ratio. MAF levels were also classified as high (> 40%), medium (15–40%) or low (