Infectious meningitis/encephalitis: Evaluation of a rapid and fully automated multiplex PCR in the microbiological diagnostic workup
- Resource Type
- Authors
- Piccirilli, Giulia; Chiereghin, Angela; Gabrielli, Liliana; Giannella, Maddalena; Squarzoni, Diego; Turello, Gabriele; Felici, Silvia; Caterina Vocale; Zuntini, Roberta; Gibertoni, Dino; Maraolo, Alberto Enrico; Ambretti, Simone; Lazzarotto, Tiziana
- Source
- Scopus-Elsevier
Web of Science
- Subject
- Adult
Automation, Laboratory
Male
Bacteria
Viruses
Humans
Female
Encephalitis, Viral
Child
Central nervous system infections, Meningitis, Encephalitis, Multiplex molecular methods, Cerebrospinal fluid
Meningitis, Viral
Multiplex Polymerase Chain Reaction
Meningitis, Bacterial
- Language
Infectious diseases of the central nervous system (CNS) such as meningitis/encephalitis (ME) require rapid identification of causative pathogens for effective treatment. This study evaluated the analytical performance and clinical utility of a fully automated multiplex PCR test to improve the microbiological diagnostic workup of ME. Seventy-seven cerebrospinal fluid (CSF) samples from 77 patients with suspected ME were studied. The samples were tested by FilmArray™ (FA) ME Panel test and the results were compared with those obtained using conventional microbiological procedures (CMP). Furthermore, the assay’s validity was evaluated testing 5 pooled CSF samples positive for different pathogens. The data showed a good concordance (90.9%) between the FA ME panel test and CMP results. Discrepant results were observed in CSF samples with low viral load (5/77) and in samples of patients (2/77) undergoing antimicrobial therapy for fungal infection. The ability of the FA ME panel test to correctly detect the target pathogens was confirmed. Faster microbiological diagnosis was obtained by the FA ME test in comparison to CMP for both bacterial and viral analytes (P