Influence of hydrodynamic pressure on chondrogenic differentiation of human bone marrow mesenchymal stem cells cultured in perfusion system
- Resource Type
- Authors
- Leila Mohammadi Amirabad; Shahab Faghihi; Masoud Soleimani; Soheila Zamanlui
- Source
- Biologicals. 56:1-8
- Subject
- Adult
Cartilage, Articular
0301 basic medicine
Adolescent
Cell Culture Techniques
Type II collagen
Bone Marrow Cells
Bioengineering
Stimulation
02 engineering and technology
Applied Microbiology and Biotechnology
Young Adult
03 medical and health sciences
chemistry.chemical_compound
Bioreactors
Chondrocytes
Downregulation and upregulation
Pressure
medicine
Humans
Aggrecans
Collagen Type II
Aggrecan
Pharmacology
Tissue Engineering
Tissue Scaffolds
General Immunology and Microbiology
Cartilage
Mesenchymal stem cell
Cell Differentiation
Mesenchymal Stem Cells
General Medicine
021001 nanoscience & nanotechnology
Chondrogenesis
Cell biology
Perfusion
PLGA
030104 developmental biology
medicine.anatomical_structure
chemistry
Hydrodynamics
Female
Stress, Mechanical
0210 nano-technology
Biotechnology
- Language
- ISSN
- 1045-1056
The natural conditions of chondrocytes in native cartilage including mechanical forces and surface topology could be simulated to enhance chondrogenesis. A perfusion system recapitulating the hydrodynamic pressure of cartilage tissue is designed. Mesenchymal stem cells (MSCs) are isolated and seeded on aligned nanofibrous PCL/PLGA scaffolds that mimic the structure of superficial zone of articular cartilage. The cell-seeded scaffolds are placed into the perfusion bioreactor and exposed to chondrogenic differentiating medium. The chondrogenesis is then investigated by histological analysis and real time PCR for cartilage-specific genes. The highest expression levels of aggrecan and type II collagen are observed in the cells cultured in the presence of differentiating medium and mechanical stimulation. The expression level of type II collagen is higher than aggrecan in presence of differentiating medium and absence of mechanical stimulation. On the contrary, the expression ratio of aggrecan is higher than type II collagen in presence of mechanical stimulation and absence of differentiating medium. These results show the dominant role of mechanical stimulation and differentiating medium on upregulated expression of aggrecan and type II collagen, respectively. The application of mechanical stimulation upon cells-seeded scaffolds could mimic superficial zone of articular cartilage tissue and increase derivation of chondrocytes from MSCs.