Detection of Panton-Valentine Leukocidin Gene in Clinical Isolates of Staphylococci at Assiut University Hospitals
- Resource Type
- Authors
- Muhamad R. Abdel Hameed; Wegdan Abdel Hameed; Sherein G. Elgendy; Alaa Thabet Hassan
- Source
- The Egyptian Journal of Medical Microbiology. 25:77-83
- Subject
- 0301 basic medicine
medicine.medical_specialty
business.industry
Incidence (epidemiology)
SCCmec
030106 microbiology
Case-control study
Leukocidin
respiratory system
biochemical phenomena, metabolism, and nutrition
bacterial infections and mycoses
Microbiology
law.invention
03 medical and health sciences
Genetic marker
law
Epidemiology
bacteria
Medicine
Panton–Valentine leukocidin
business
Polymerase chain reaction
- Language
- ISSN
- 1110-2179
Background: Panton-Valentine leukocidin (PVL) toxin is mainly associated with necrotic lesions involving the skin or mucosa .PVL has been linked by epidemiological studies to community-associated methicillin resistant Staphylococci (CA-MRSA) and a relatively few data about the incidence of this toxin in nosocomial infections . Objectives: This study aimed to investigate the incidence of PVL gene in nosocomial staphylococcal isolates at Assiut University Hospitals, and to investigate its association with various risk factors and different types of infections. Methodology: This study was a case control study carried on seventy eight patients with nosocomial infections admitted to Postoperative ICU, Trauma ICU, Chest ICU and Internal Medicine ICU; also 27 patients of community acquired infections were also included as a control group. The detection of the mecA gene and PVL gene were done by single target polymerase chain reaction (PCR). Result: The PVL-gene producing strains constituted (17.9%) of all nosocomial isolates. The PVL-gene were detected in all CA-MRSA (44.4%) and not detected in CA-MSSA. Conclusion: Detection of PVL-gene in both community and hospital isolates made this gene not a reliable genetic marker for CA-MRSA.