Association of concentrations of beta-carotene in plasma on pregnancy per artificial insemination and pregnancy loss in lactating Holstein cows
- Resource Type
- Authors
- A.M.L. Madureira; José Luiz Moraes Vasconcelos; T.G. Guida; Ronaldo L.A. Cerri; S.E. Wagner; Ky G Pohler
- Source
- Web of Science
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
- Subject
- medicine.medical_treatment
Pregnancy associated glycoprotein
Single step
Pregnancy-associated-glycoprotein
03 medical and health sciences
0302 clinical medicine
Animal science
Food Animals
Pregnancy
beta-Carotene
Animals
Lactation
Medicine
Small Animals
Insemination, Artificial
030219 obstetrics & reproductive medicine
Equine
business.industry
Artificial insemination
Pregnancy Outcome
0402 animal and dairy science
food and beverages
04 agricultural and veterinary sciences
Abortion, Veterinary
beta Carotene
medicine.disease
Milk production
040201 dairy & animal science
Dairying
Beta-carotene
Body Constitution
Pregnancy, Animal
Cattle
Female
Animal Science and Zoology
business
Pregnancy/AI
- Language
- ISSN
- 0093-691X
1816-8000
Made available in DSpace on 2020-12-10T19:45:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-15 DSM Nutritional Products (Sao Paulo, Brazil) The objective of this study was to determine the association of beta-carotene concentration in plasma at the moment of artificial insemination (AI) on pregnancy/AI in lactating Holstein cows. A total of 399 events from 364 lactating Holstein cows were enrolled in the trial (143 primiparous and 221 multiparous). AIl cows were assigned to a timed AI protocol based on estradiol and progesterone. Blood samples were collected at the moment of AI and at 24 and 31d post-AI (samples on 31 d post-AI were collected only from cows that were diagnosed pregnant). The BCS were recorded at the time of AI. Plasma betacarotene was quantified from blood samples taken at the time of AI using a single step denaturation and extraction into a solvent, followed by measurement using a portable spectrophotometer. Pregnancy-associated glycoproteins (PAG) were analyzed in blood samples taken at 24 and 31 d post-AI of pregnant cows. Milk production was collected for the entire experimental period. Pregnancy diagnosis was performed by ultrasound 31 and 60 d post-AL Data was analyzed using the MIXED and GLIMMIX procedures of SAS. Cows classified as thin (