Additional file 7: of Immunohistochemical phenotyping of T cells, granulocytes, and phagocytes in the muscle of cancer patients: association with radiologically defined muscle mass and gene expression
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- Anoveros-Barrera, Ana; Amritpal Bhullar; Stretch, Cynthia; Dunichand-Hoedl, Abha; Martins, Karen; Rieger, Aja; Bigam, David; McMullen, Todd; Bathe, Oliver; Putman, Charles; Field, Catherine; Baracos, Vickie; Mazurak, Vera
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Figure S2. Immunostaining of serial cross-sections of muscle tissue: CD11b+CD14+CD15+ cells (A) and laminin-dystrophin (B). Stained nuclei in blue. A.1 Original image with no brightness manipulation. A.2 and A.3 Brightness was increased to visually appreciate the location of the CD11b+CD14+CD15+ cell (arrow) on the endomysial area. B. Serial cross-section used to confirm the location of immune cells on the periphery of muscle fibers (endomysium). Asterisks mark muscle fibers used as a reference point, and immune cell location is pointed by the white arrow. Scale bar 11â Îźm. (DOCX 1549 kb)