Effect of shearing stress on the radial heterogeneity and chromatographic performance of styrene-based polymerised high internal phase emulsions prepared in capillary format
- Resource Type
- Authors
- Stefan Antonius Franciscus Bon; R. Dario Arrua; Emily F. Hilder; Christopher T. Desire; Fotouh R. Mansour
- Source
- Subject
- Void (astronomy)
Materials science
radial heterogeneity
Capillary action
General Chemical Engineering
02 engineering and technology
010402 general chemistry
01 natural sciences
Styrene
chemistry.chemical_compound
Pulmonary surfactant
void size distribution
chromatographic performance
Shear stress
poly(styrene-co-divinylbenzene)
shearing stress
ostwald ripening
Curing (chemistry)
Chromatography
General Chemistry
021001 nanoscience & nanotechnology
0104 chemical sciences
high internal phase emulsions
chemistry
Polymerization
Emulsion
internal diameters
capillary format
0210 nano-technology
- Language
- English
Poly(styrene-co-divinylbenzene) monoliths were prepared from the polymerisation of water-in-monomer high internal phase emulsions consisting of a 90 vol% internal phase and stabilised by the non-ionic surfactant Span 80®. The materials were prepared in capillary housings of various internal diameters ranging from 150 mm to 540 mm by simply passing the emulsion through the capillaries. When low shear(300 rpm) was used for emulsification, the droplet and resulting void size distributions were observed to shift towards lower values when the emulsions were forced through capillaries of internal diameter less than 540 mm and all columns exhibited significant radial heterogeneity. When high shear was employed(14 000 rpm) the resulting emulsions preserved their structure when forced through these capillaries and possessed narrower void size distributions with no obvious radial heterogeneity observed upon curing.This resulted in significantly improved chromatographic performance for the separation of a standard mixture of proteins when compared to the materials prepared under low shear. Refereed/Peer-reviewed