Hsa_circ_0016760 exacerbates the malignant development of non-small cell lung cancer by sponging miR-145-5p/FGF5
- Resource Type
- Authors
- Kai Yuan; Zheng Zhu; Jichun Tong; Bin Zhong; Qiyong Wu; Ming Zhang
- Source
- Oncology Reports
- Subject
- Male
Cancer Research
Lung Neoplasms
Carcinogenesis
Cell
Apoptosis
Kaplan-Meier Estimate
Mice
Cell Movement
Carcinoma, Non-Small-Cell Lung
Pneumonectomy
Lung
Chemistry
General Medicine
Articles
Middle Aged
Cell cycle
Prognosis
Up-Regulation
Gene Expression Regulation, Neoplastic
medicine.anatomical_structure
Real-time polymerase chain reaction
Oncology
Female
miR-145-5p/FGF5
Fibroblast Growth Factor 5
in vivo study
Downregulation and upregulation
Cell Line, Tumor
medicine
Animals
Humans
Gene silencing
Neoplasm Invasiveness
Gene Silencing
hsa_circ_0016760
non-small cell lung cancer
Aged
Cell Proliferation
Oncogene
Cancer
RNA, Circular
medicine.disease
Xenograft Model Antitumor Assays
Molecular medicine
respiratory tract diseases
MicroRNAs
Cancer research
progression
Follow-Up Studies
- Language
- English
- ISSN
- 1791-2431
1021-335X
Hsa_circ_0016760 expression has been reported to be increased in non‑small cell lung cancer (NSCLC). The present study was designed to explore the role and mechanism of hsa_circ_0016760 in regulating NSCLC progression. In total, 60NSCLC patients were followed‑up for 60 months after surgery. Hsa_circ_0016760 expression in tumor tissues and adjacent non‑tumor tissues of NSCLC patients was explored by reverse transcription quantitative polymerase chain reaction (RT‑qPCR). NSCLC cell proliferation was monitored by CCK‑8 assay and EdU experiment. Transwell assays were used for the detection of NSCLC cell migration and invasion. The target of hsa_circ_0016760 (or miR‑145‑5p) was validated by luciferase reporter gene assay and RNA immunoprecipitation experiment. A xenograft model was studied with nude mice. Immunohistochemical staining was applied for the detection of Ki67 expression in xenograft tumors. Hsa_circ_0016760/miR‑145‑5p/FGF5 expression in tissues and cells was investigated by RT‑qPCR and western blotting. Hsa_circ_0016760 was aberrantly upregulated in NSCLC, which was associated with poor prognosis of patients (P