MMP-2 as an early synovial biomarker for cranial cruciate ligament disease in dogs
- Resource Type
- Authors
- Richard Danger; L. Boland; Sophie Brouard; Olivier Gauthier; Q. Cabon; B. Bouvy; M. Rabillard
- Source
- Veterinary and Comparative Orthopaedics and Traumatology
Veterinary and Comparative Orthopaedics and Traumatology, 2014, 27 (03), pp.210-215. ⟨10.3415/VCOT-13-06-0082⟩
Veterinary and Comparative Orthopaedics and Traumatology, Schattauer, 2014, 27 (03), pp.210-215. ⟨10.3415/VCOT-13-06-0082⟩
- Subject
- musculoskeletal diseases
Pathology
medicine.medical_specialty
040301 veterinary sciences
Matrix metalloproteinase
Gene Expression Regulation, Enzymologic
C-reactive protein
0403 veterinary science
Cruciate ligament
03 medical and health sciences
0302 clinical medicine
Dogs
Synovial Fluid
contra-lateral rupture
medicine
Synovial fluid
Animals
Clinical significance
Dog Diseases
Anterior Cruciate Ligament
030203 arthritis & rheumatology
Rupture
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology
General Veterinary
biology
business.industry
Anterior Cruciate Ligament Injuries
Significant difference
matrix metalloproteinases
04 agricultural and veterinary sciences
Stifle
medicine.anatomical_structure
Matrix Metalloproteinase 9
dog
biology.protein
Prospective clinical study
Biomarker (medicine)
Matrix Metalloproteinase 2
Animal Science and Zoology
business
Cranial cruciate ligament disease
Biomarkers
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
- Language
- English
- ISSN
- 0932-0814
SummaryObjectives: To measure the activity of matrix metalloproteinases (MMP)-2 and -9 in synovial fluid from the stifle joints of dogs with cranial cruciate ligament (CrCL) rupture and to compare that to values from contralateral stifle joints and dogs with clinically normal stifle joints. Additionally, the C-reactive protein (CRP) levels were also measured.Methods: Fourteen large breed dogs with unilateral CrCL rupture and 11 large breed normal dogs were included in this prospective clinical study. Synovial fluid was collected from CrCL-ruptured stifle joints, contralateral clinically normal stifle joints of the same dogs, and stifle joints of normal dogs. Serum was also collected. Synovial fluid activities of MMP-2 and MMP-9 and serum CRP level were measured.Results: The MMP-2 activity in synovial fluid was significantly higher in CrCL-ruptured joints compared to contralateral joints and to stifles from normal dogs. There was no significant difference in activity of MMP-2 in contralateral joints of CrCL-ruptured dogs compared to normal dogs. Both serum CRP level and MMP-9 activity did not differ significantly between the studied conditions.Clinical significance: It was confirmed that MMP-2 activity is significantly related to CrCL rupture, but there was a failure to demonstrate any significant increase in the contralateral joints compared to the stifle joints of normal dogs. The MMP-2 involvement in progressing CrCL disease still has to be defined.