Role of the RNA/DNA kinase Grc3 in transcription termination by RNA polymerase I
- Resource Type
- Authors
- Javier Martinez; Nick J. Proudfoot; Priscilla Braglia; Alexander Schleiffer; Katrin Heindl
- Source
- EMBO Reports
- Subject
- Polynucleotide 5'-Hydroxyl-Kinase
Saccharomyces cerevisiae Proteins
Transcription, Genetic
kinase
Termination factor
RNA-dependent RNA polymerase
RNA polymerase II
Saccharomyces cerevisiae
Biology
Biochemistry
DNA, Ribosomal
03 medical and health sciences
0302 clinical medicine
Transcription (biology)
RNA Polymerase I
Genetics
RNA polymerase I
Phosphorylation
RNA Processing, Post-Transcriptional
Molecular Biology
Polymerase
030304 developmental biology
transcription termination
Terminator Regions, Genetic
0303 health sciences
Pol I
Scientific Reports
DNA Polymerase I
Molecular biology
Terminator (genetics)
Antitermination
biology.protein
Grc3
030217 neurology & neurosurgery
- Language
- English
- ISSN
- 1469-3178
1469-221X
Role of the RNA/DNA kinase Grc3 in transcription termination by RNA polymerase I GRC3 of S.cerevisiae encodes a polynucleotide kinase that is required for efficient termination of transcription by RNA polymerase I. This study proposes that GRC3 acts to maintain the 5′-phosphate end of the downstream Rnt1 cleavage product thus allowing Rat1 to efficiently degrade this RNA and so “torpedo” RNA polymerase I.
Transcription termination by RNA polymerase I in Saccharomyces cerevisiae is mediated by a ‘torpedo' mechanism: co-transcriptional RNA cleavage by Rnt1 at the ribosomal DNA 3′-region generates a 5′-end that is recognized by the 5′–3′ exonuclease Rat1; this degrades the downstream transcript and eventually causes termination. In this study, we identify Grc3 as a new factor involved in this process. We demonstrate that GRC3, an essential gene of previously unknown function, encodes a polynucleotide kinase that is required for efficient termination by RNA polymerase I. We propose that it controls the phosphorylation status of the downstream Rnt1 cleavage product and thereby regulates its accessibility to the torpedo Rat1.