Sequence alignment of HAP1 family proteins. ClustalO alignment of HAP1 (human, rat, dog), TRAK1 (human, rat, dog), TRAK2 (human, rat, dog), T27A3.1 (C. elegans) and Milton (Drosophila) proteins is shown. The red boxed region indicates the HAP1_N domain, and the purple boxed region indicates the Milt/TRAK domain. Amino acid color identifies residues that are small/hydrophobic/aromatic (red), acidic (blue) basic (magenta) and hydroxyl/sulfhydryl/amine/glycine (green). Degree of conservation is indicated by an asterisk (*) for complete agreement, a colon (:) for conservation between groups of strongly similar properties, and a dot (.) for conservation between groups of weakly similar properties. Sequence identifiers are as indicated in Materials and methods. (PPTX 3364 kb)