Background: Stigma is present during the lifespan of individuals with cystic fibrosis (CF); consequently, instruments to assess this psychosocial aspect are necessary. Few instruments are validated and adapted to Brazilian Portuguese. We aimed to translate, cross-culturally adapt, and evaluate psychometric properties of the CF stigma scale. Methods: We conducted an exploratory study of cross-cultural adaptation involving translation, back translation, revision by an expert committee, and a pre-test. Psychometric properties (content validity, test-retest reliability, and convergent validity) were analyzed based on the adapted version of the scale and responded by 52 Brazilian individuals with CF older than 18 years. Results: Translation and cross-cultural adaptation obtained kappa indexes higher than 0.61 on the expert committee phase and between 0.48 and 0.72 on pre-test. The Brazilian version of CF stigma scale showed excellent psychometric properties: i) internal consistency, α = 0.836; ii) mean correlation between items and test-retest: r = 0.886, p < 0.0001; and iii) convergent validity, CF stigma scale correlated positively with anxiety scale and negatively with general and specific scores of quality of life in CF. Conclusion: CF stigma scale was adequately translated and cross-culturally adapted for the Brazilian population. Psychometric properties of the Brazilian version favor its use in future studies regarding stigma conducted with Brazilian individuals with CF.