Effects of conformation and hydrogen bonding on the CC and NCN stretching Raman bands of N-deuterated histidinium
- Resource Type
- Authors
- Hideo Takeuchi; Hirotsugu Hiramatsu; Noriko Miki
- Source
- Journal of Raman Spectroscopy. 41:1708-1713
- Subject
- Quantitative Biology::Biomolecules
Hydrogen
Stereochemistry
Hydrogen bond
chemistry.chemical_element
Acceptor
chemistry.chemical_compound
Crystallography
symbols.namesake
chemistry
Deuterium
symbols
Imidazole
Wavenumber
General Materials Science
Raman spectroscopy
Spectroscopy
Histidine
- Language
- ISSN
- 0377-0486
Histidine is an important and versatile amino acid residue that plays a variety of structural and functional roles in proteins. Although the Raman bands of histidine are generally weak, histidine in the N-deuterated cationic form with imidazole NπD and NτD bonds (N-deuterated histidinium) gives two strong Raman bands assignable to the C4C5 stretch (νCC) and the NπC2Nτ symmetric stretch (νNCN) of the imidazole ring. We examined the Raman spectra of N-deuterated histidinium in 12 crystals with known structures. The observed νCC and νNCN wavenumbers were analyzed to find empirical correlations with the conformation and hydrogen bonding. The effect of conformation on the vibrational wavenumber was expressed as a threefold cosine function of the CαCβC4C5 torsional angle. The effect of hydrogen bonding at Nπ or Nτ was assumed to be proportional to the inverse sixth power of the distance between the hydrogen and acceptor atoms. Multiple linear regression analysis clearly shows that the conformational effect on the vibrational wavenumber is comparable for νCC and νNCN. The hydrogen bond at Nπ weakly lowers the νCC wavenumber and substantially raises the νNCN wavenumber. On the other hand, the hydrogen bond at Nτ strongly raises the νCC wavenumber but does not affect the νNCN wavenumber. These empirical correlations may be useful in Raman spectral analysis of the conformation and hydrogen bonding states of histidine residues in proteins. Copyright © 2010 John Wiley & Sons, Ltd.