Correlation between cell morphology and aggrecan gene expression level during differentiation from mesenchymal stem cells to chondrocytes
- Resource Type
- Authors
- Satoru Koizumi; Shin Ichi Kondo; Hiroshi Masuhara; Yoichiroh Hosokawa; Misawa Hiroaki; Shigeyuki Wakitani; Takayuki Kitabayashi; Mutsumi Takagi; Masashi Fujiwara; Haruka Hirose; Kosei Ueno
- Source
- Biotechnology letters. 30(7)
- Subject
- Fetal Proteins
Mesenchyme
Cell Adhesion Molecules, Neuronal
Bioengineering
Biology
Cell morphology
Applied Microbiology and Biotechnology
Chondrocyte
Chondrocytes
Antigens, CD
medicine
Humans
CD90
Aggrecans
RNA, Messenger
Cell adhesion
Cell Shape
Aggrecan
Mesenchymal stem cell
Cell Differentiation
Mesenchymal Stem Cells
General Medicine
Cell biology
medicine.anatomical_structure
Gene Expression Regulation
Immunology
Thy-1 Antigens
Stem cell
Biotechnology
- Language
- ISSN
- 0141-5492
A morphological parameter of polygonal index was defined as the ratio of cell adhesion area versus the square of the major cell axis, and cells that had an adhesion area larger than 4000 mum(2) and a polygonal index larger than 0.3 were considered large polygonal cells. Cell morphology tended to change from fibroblast-like to polygonal and the percentage of the large polygonal cells increased almost in proportion to aggrecan mRNA expression level during the differentiation culture of mesenchymal stem cells (MSCs) to chondrocytes. Approximately 80% of the large polygonal cells were negative for MSC marker (CD90, CD166) expression and the aggrecan mRNA expression level of the large polygonal cells was markedly higher than that of cells with other morphologies.