Influence of the OGG1 Ser326Cys polymorphism on oxidatively damaged DNA and repair activity
- Resource Type
- Authors
- Annie Aarup Jensen; Peter Møller; Elad Dorry; Mille Løhr; Louise Eriksen; Morten Grønbæk; Steffen Loft
- Source
- Jensen, A A, Løhr, M, Eriksen, L, Grønbæk, M, Dorry, E, Loft, S & Møller, P F 2012, ' Influence of the OGG1 Ser326Cys polymorphism on oxidatively damaged DNA and repair activity ', Free Radical Biology & Medicine, vol. 52, no. 1, pp. 118-25 . https://doi.org/10.1016/j.freeradbiomed.2011.09.038
- Subject
- Adult
Male
Guanine
Lung Neoplasms
DNA Repair
Genotype
DNA damage
DNA repair
Denmark
Adenocarcinoma of Lung
Adenocarcinoma
Biology
Polymorphism, Single Nucleotide
Biochemistry
DNA Glycosylases
MUTYH
Physiology (medical)
Humans
Genetic Predisposition to Disease
Aged
Homozygote
Formamidopyrimidine DNA glycosylase
Middle Aged
Molecular biology
Comet assay
Oxidative Stress
DNA glycosylase
Case-Control Studies
Leukocytes, Mononuclear
Female
Comet Assay
ERCC1
Oxidation-Reduction
DNA Damage
- Language
- ISSN
- 0891-5849
Oxidatively damaged DNA base lesions are considered to be mainly repaired by 8-oxoguanine DNA glycosylase (OGG1) mediated pathways. We investigated the effect of the OGG1 Ser326Cys polymorphism on the level and repair of oxidatively damaged DNA in mononuclear blood cells (MNBC) by means of the comet assay. We collected blood samples from 1,019 healthy subjects and genotyped for the OGG1 Ser326Cys polymorphism. We found 49 subjects homozygous for the variant genotype (Cys/Cys) and selected same numbers of age-matched subjects with the heterozygous (Ser/Cys) and homozygous wild-type genotype (Ser/Ser). Carriers of the Cys/Cys genotype had higher levels of formamidopyrimidine DNA glycosylase (FPG) sensitive sites in MNBC (0.31 ± 0.03 lesions/10(6)bp) compared to Ser/Ser (0.19 ± 0.02 lesions/10(6)bp, P