Simultaneous conduction mapping and intracellular membrane potential recording in isolated atria
- Resource Type
- Authors
- Hany Dimitri; David A. Saint; W. Lim; David G Morris; Melissa Neo; Dennis H. Lau; Pawel Kuklik; Prashanthan Sanders
- Source
- Canadian Journal of Physiology and Pharmacology. 94:563-569
- Subject
- Male
0301 basic medicine
Physiology
Neural Conduction
Action Potentials
Pilot Projects
Stimulation
In Vitro Techniques
030204 cardiovascular system & hematology
Nerve conduction velocity
Membrane Potentials
Rats, Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
Tachycardia
Physiology (medical)
Animals
Heart Atria
Pharmacology
Membrane potential
Chemistry
Functional Neuroimaging
Reproducibility of Results
Arrhythmias, Cardiac
Intracellular Membranes
General Medicine
Multielectrode array
Anatomy
Atrial Function
Microarray Analysis
Microelectrode
Electrophysiology
030104 developmental biology
Biophysics
Electrophysiologic Techniques, Cardiac
Microelectrodes
Intracellular
- Language
- ISSN
- 1205-7541
0008-4212
We describe a novel approach for simultaneously determining regional differences in action potential (AP) morphology and tissue electrophysiological properties in isolated atria. The epicardial surface of rat atrial preparations was placed in contact with a multi-electrode array (9 × 10 silver chloride electrodes, 0.1 mm diameter and 0.1 mm pitch). A glass microelectrode (100 MΩ) was simultaneously inserted into the endocardial surface to record intracellular AP from either of 2 regions (A, B) during pacing from 2 opposite corners of the tissue. AP duration at 80% of repolarisation and its restitution curve was significantly different only in region A (p < 0.01) when AP was initiated at different stimulation sites. Alternans in AP duration and AP amplitude, and in conduction velocity were observed during 2 separate arrhythmic episodes. This approach of combining microelectrode array and intracellular membrane potential recording may provide new insights into arrhythmogenic mechanisms in animal models of cardiovascular disease.