Transposon mutagenesis in a hyper-invasive clinical isolate of Campylobacter jejuni reveals a number of genes with potential roles in invasion
- Resource Type
- Authors
- Duncan J. Maskell; Mary C. Bagnall; Muhammad Afzal Javed; Andrew J. Grant; Georgina Manning; Diane G. Newell
- Source
- Microbiology (Reading, England). 156(Pt 4)
- Subject
- Transposable element
Genetics
biology
Base Sequence
Virulence
Mutant
Molecular Sequence Data
Mutagenesis (molecular biology technique)
biology.organism_classification
Sleeping Beauty transposon system
Microbiology
Genome
Campylobacter jejuni
Mutagenesis, Insertional
Bacterial Proteins
Campylobacter Infections
Mutation
DNA Transposable Elements
Humans
Transposon mutagenesis
Gene
- Language
- ISSN
- 1465-2080
1350-0872
Transposon mutagenesis has been applied to a hyper-invasive clinical isolate ofCampylobacter jejuni, 01/51. A random transposon mutant library was screened in anin vitroassay of invasion and 26 mutants with a significant reduction in invasion were identified. Given that the invasion potential ofC. jejuniis relatively poor compared to other enteric pathogens, the use of a hyper-invasive strain was advantageous as it greatly facilitated the identification of mutants with reduced invasion. The location of the transposon insertion in 23 of these mutants has been determined; all but three of the insertions are in genes also present in the genome-sequenced strain NCTC 11168. Eight of the mutants contain transposon insertions in one region of the genome (∼14 kb), which when compared with the genome of NCTC 11168 overlaps with one of the previously reported plasticity regions and is likely to be involved in genomic variation between strains. Further characterization of one of the mutants within this region has identified a gene that might be involved in adhesion to host cells.