Contains additional information methods data associated with accompanying supplemental figures, and additional data on in vivo studies, including information on experimental animals, housing and sample size. Contains 4 supplemental figures demonstrating: Figure 1, the cytotoxicity of clodronate liposomes against macrophages and tumor cells in vitro; Figure 2, that depletion of DC abrogates the priming of tumor-antigen specific CD8 T-cells following radiation and anti-CD40 mAb therapy; Figure 3, the impact of depletion of CD169 macrophages on radiation and anti-CD40 mAb therapy; Figure 4, combination radiation and R848 (TLR-7 agonist)leads to priming of tumor-specific CD8 T-cells.