Mapping Intracellular Temperature Using Green Fluorescent Protein
- Resource Type
- Authors
- Sebastian Thompson; Jon S. Donner; Romain Quidant; Mark P. Kreuzer; Guillaume Baffou
- Source
- Nano Letters
Nano Letters, 2012, 12, pp.2107-2111. ⟨10.1021/nl300389y⟩
Nano Letters, American Chemical Society, 2012, 12, pp.2107-2111. ⟨10.1021/nl300389y⟩
- Subject
- Fluorescence-lifetime imaging microscopy
Thermal imaging
Cell division
Surface Properties
Green Fluorescent Proteins
Metal Nanoparticles
Bioengineering
Nanotechnology
anisotropy
02 engineering and technology
GFP
010402 general chemistry
01 natural sciences
Green fluorescent protein
fluorescence imaging
Cell Line, Tumor
Humans
General Materials Science
Particle Size
Mechanical Engineering
Temperature
General Chemistry
Photothermal therapy
Photochemical Processes
021001 nanoscience & nanotechnology
Condensed Matter Physics
Fluorescence
3. Good health
0104 chemical sciences
Cancer cell
cancer cells
[SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic
Biophysics
Gold
0210 nano-technology
Intracellular
Fluorescence anisotropy
HeLa Cells
- Language
- ISSN
- 1530-6992
1530-6984
International audience; Heat is of fundamental importance in many cellular processes such as cell metabolism, cell division and gene expression. Accurate and noninvasive monitoring of temperature changes in individual cells could thus help clarify intricate cellular processes and develop new applications in biology and medicine. Here we report the use of green fluorescent proteins (GFP) as thermal nanoprobes suited for intracellular temperature mapping. Temperature probing is achieved by monitoring the fluorescence polarization anisotropy of GFP. The method is tested on GFP- transfected HeLa and U-87 MG cancer cell lines where we monitored the heat delivery by photothermal heating of gold nanorods surrounding the cells. A spatial resolution of 300 nm and a temperature accuracy of about 0.4 °C are achieved. Benefiting from its full compatibility with widely used GFP-transfected cells, this approach provides a noninvasive tool for fundamental and applied research in areas ranging from molecular biology to therapeutic and diagnostic studies.