Biophysical study on the interaction of cartap hydrochloride and hemoglobin: Heme degradation and functional changes of protein
- Resource Type
- Authors
- Parvaneh Maghami; Mahdieh Emadi; Khatereh Khorsandi; Reza Hosseinzadeh
- Source
- Journal of Biochemical and Molecular Toxicology. 33
- Subject
- 0301 basic medicine
Insecticides
Hydrochloride
Health, Toxicology and Mutagenesis
Heme
Toxicology
Hemolysis
Biochemistry
Hemoglobins
03 medical and health sciences
Heme degradation
Human health
chemistry.chemical_compound
0302 clinical medicine
Tetramer
Thiocarbamates
Animals
Humans
Molecular Biology
Cartap
030102 biochemistry & molecular biology
Oxygen transport
General Medicine
Structure and function
chemistry
030220 oncology & carcinogenesis
Proteolysis
Biophysics
Molecular Medicine
Hemoglobin
- Language
- ISSN
- 1099-0461
1095-6670
Cartap hydrochloride is a mildly perilous insecticide known as "Padan" which is used largely in agricultural farms to control weevil and caterpillars. The over use of cartap causes harmful effects on human health. Since the blood may acts as a target and carrier for insecticides, the effect of these compounds on blood in mammalian toxicology is very important. Hemoglobin is a tetramer protein that play critical role in oxygen transport. The aim of this study was to analyze and compare the function and structural changes of hemoglobin in the presence of different concentrations of cartap by employing different spectroscopic techniques. The obtained results show that cartap has a high hemolytic effect which is increased with cartap concentration and reduces the thermal midpoint of hemoglobin. Fluorescence measurements reveal heme degradation at different concentrations of cartap. In consequence of theoretical and experimental results, cartap has an undesirable effect on hemoglobin structure and function.