Figure S2. (Arg)9-GST SH2 TrM could effectively capture diverse pY proteins in A375 and A375/DDP cells. Levels of pY proteins from A375(a) and A375/DDP(b) cells stimulated with or without EGF (100 ng/ml) were assessed with Anti-pY antibody. The whole cell lysates were incubated with (Arg)9-GST, (Arg)9-GST SH2 Wt or (Arg)9-GST SH2 TrM for 12 h at 4 °C and purified by an appropriate amount of glutathione agarose. Data shown are representative of three independent experiments. (PPTX 107 kb)