Molecular strategies to increase the levels of heterologous transcripts in Komagataella phaffii for protein production
- Resource Type
- Authors
- Maritza Ocampo Bentacur; Fernando Araripe Gonçalves Torres; Lidia Maria Pepe de Moraes; Janice Lisboa De Marco; Luiza Cesca Piva; Viviane Castelo Branco Reis
- Source
- Subject
- 0301 basic medicine
Gene Dosage
Heterologous
Bioengineering
Biology
Protein Engineering
Applied Microbiology and Biotechnology
Gene dosage
Pichia pastoris
03 medical and health sciences
Ascomycota
Gene Expression Regulation, Fungal
Gene expression
Cloning, Molecular
Promoter Regions, Genetic
Transcription factor
Gene
Genetics
General Medicine
Protein engineering
biology.organism_classification
Recombinant Proteins
030104 developmental biology
Genetic Enhancement
Commentary
Heterologous expression
Biotechnology
Transcription Factors
- Language
- English
Komagataella phaffii (formerly Pichia pastoris) is a well-known fungal system for heterologous protein production in the context of modern biotechnology. To obtain higher protein titers in this system many researchers have sought to optimize gene expression by increasing the levels of transcription of the heterologous gene. This has been typically achieved by manipulating promoter sequences or by generating clones bearing multiple copies of the desired gene. The aim of this work is to describe how these different molecular strategies have been applied in K. phaffii presenting their advantages and drawbacks.