Effects of granulocyte-colony-stimulating factor on Monosomy 7 aneuploidy in healthy hematopoietic stem cell and granulocyte donors
- Resource Type
- Authors
- Matthew J, Olnes; Andrea, Poon; Susan J, Miranda; Loretta, Pfannes; Zachary, Tucker; Kelsey, Loeliger; Hesed, Padilla-Nash; Yu Ying, Yau; Thomas, Ried; Susan F, Leitman; Neal S, Young; Elaine M, Sloand
- Source
- Transfusion. 52(3)
- Subject
- Hematopoietic Stem Cell Transplantation
Aneuploidy
Hematopoietic Stem Cells
Dexamethasone
Genomic Instability
Hematopoietic Stem Cell Mobilization
Tissue Donors
Article
Granulocyte Colony-Stimulating Factor
Humans
Chromosome Deletion
Glucocorticoids
Chromosomes, Human, Pair 7
Granulocytes
- Language
- ISSN
- 1537-2995
Reports of Monosomy 7 in patients receiving granulocyte-colony-stimulating factor (G-CSF) have raised concerns that this cytokine may promote genomic instability. However, there are no studies addressing whether repeated administration of G-CSF produces Monosomy 7 aneuploidy in healthy donors.We examined Chromosomes 7 and 8 by fluorescent in situ hybridization (FISH) in CD34+ cells from 35 healthy hematopoietic stem cell transplant (HSCT) donors after G-CSF administration for 5 days and by spectral karyotyping analysis (SKY) in four individuals to assess chromosomal integrity. We also studied 38 granulocyte donors who received up to 42 doses of G-CSF and dexamethasone (Dex) using FISH for Chromosomes 7 and 8.We found no abnormalities in Chromosomes 7 and 8 in G-CSF-mobilized CD34+ cells when assessed by FISH or SKY, nor did we detect aneuploidy in G-CSF- and Dex-treated donors.G-CSF does not promote clinically detectable Monosomy 7 or Trisomy 8 aneuploidy in HSCT or granulocyte donors. These findings should be reassuring to healthy HSCT and granulocyte donors.