Additional file 4: Supplementary Figure 4. IDH1 knockout suppresses tumor growth in vivo. A] Relative mRNA levels, normalized to mRNA levels of 18S; Western blot analysis of IDH1 expression after IDH1 knockout by CRISPR/Cas9 (IDH1.KO) compared to control (IDH1.EV) A375 cells. B] Mice were injected with IDH1.EV and IDH1.KO A375 cells (n = 5 per group) and tumor sizes were monitored for 5 weeks. Images of tumors at the end of the experiment are shown. C] Tumor volumes of IDH1.EV and IDH1.KO A375 xenografts. D] Histograms show tumor volumes with IDH1.EV and IDH1.KO A375 xenografts at the end of the experiment. E] Relative glucose levels in adjacent skin and xenograft. F] Relative free magnesium levels in skin, xenografts and serum. Each data point represents the mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.