Additional file 5: Supplementary Figure 5. In vitro response of melanoma cells to treatment with TMZ in combination with AG-120. A] Silencing IDH1 followed by treatment with TMZ for 5 days under high glucose (25 mM) and B] low glucose (2.5 mM) in SK-MEL-28 cells; IC50 values are provided. C] Relative ROS levels after 48 hours in A375 cell line under low glucose combined with TMZ and AG-120. D] Relative ROS levels after 48 hours in SK-MEL-28 cells under low glucose combined with TMZ and AG-120. E] Cell viability of SK-MEL-28 cells, treated with the indicated doses of TMZ; IC50 values are provided. F] Cell viability of SK-MEL-28 cells treated with indicated doses of AG-120 for 6 days. IC50 values are provided. G] Drug sensitivity in SK-MEL-28 cells under low glucose, with varying concentrations of TMZ and AG-120, cultured for 5 days under low glucose. IC50 results are provided. H] Drug matrix heatmap 5 × 8 (AG-120 and TMZ) grid showing percent viability and Bliss Independence scores in SK-MEL-28 cells cultured under 2.5 mM glucose for 5 days. Positive values reflecting synergism appear green on the heatmap (Bliss volume ≥ 10). All treatments with AG-120 were carried under low glucose (2.5 mM) and low Mg2+ (0.08 mM).