Genomic characterization of IncpA1763-KPC: IncFIIK7 type plasmids p13294-KPC and pA1966-NR from Klebsiella pneumoniae
- Resource Type
- Authors
- Fei Zhai; Qifeng Sun; Rongwu Xiang; Liang Luhua; Yigang Tong; Jiao Feng
- Source
- Future Microbiology. 15:713-721
- Subject
- 0301 basic medicine
Microbiology (medical)
Genetics
Klebsiella pneumoniae
030106 microbiology
Drug resistance
Biology
biology.organism_classification
Microbiology
Phenotype
03 medical and health sciences
030104 developmental biology
Antibiotic resistance
Plasmid
Mobile genetic elements
Insertion sequence
Gene
- Language
- ISSN
- 1746-0921
1746-0913
Aim: To characterize two plasmids p13294-KPC and pA1966-NR from clinical Klebsiella pneumoniae strains. Materials & methods: Plasmids p13294-KPC and pA1966-NR were fully sequenced and then detailed genomic analysis was performed in this work. The antimicrobial resistance phenotypes were determined. Results: p13294-KPC and pA1966-NR displayed IncpA1763-KPC:IncFIIK7 dual-replicon structures. The backbone of these two plasmids were closely related to each other. p13294-KPC contained two accessory modules, namely ΔIS Kpn25 and blaKPC-2 region, and the blaKPC-2 region carried a range of mobile elements and resistance gene blaKPC-2. while pA1966-NR contained four individual IS elements in its backbone and carried no resistance genes. Conclusion: This study provided a deeper insight into the genomic characterization of IncpA1763-KPC: IncFIIK7 type plasmids p13294-KPC and pA1966-NR.