Rhesus rhadinovirus infection in healthy and SIV-infected macaques at Tulane National Primate Research Center
- Resource Type
- Authors
- Laura S. Levy; Gary B. Baskin; Kristin R Ruff; Laura Simpson; Michael Murphey-Corb
- Source
- Journal of Medical Primatology. 32:1-6
- Subject
- Male
Rhadinovirus
Lymphoma
animal diseases
viruses
Simian Acquired Immunodeficiency Syndrome
medicine.disease_cause
Polymerase Chain Reaction
complex mixtures
Peripheral blood mononuclear cell
biology.animal
medicine
Animals
Primate
Longitudinal Studies
General Veterinary
biology
Monkey Diseases
virus diseases
Herpesviridae Infections
Viral Load
Simian immunodeficiency virus
medicine.disease
biology.organism_classification
Macaca mulatta
Virology
Peripheral blood
Tumor Virus Infections
Real-time polymerase chain reaction
DNA, Viral
Immunology
Female
Simian Immunodeficiency Virus
Animal Science and Zoology
- Language
- ISSN
- 1600-0684
0047-2565
Rhesus rhadinovirus (RRV) infection was quantified in peripheral blood mononuclear cells (PBMC) from healthy and simian immunodeficiency virus (SIV)-infected rhesus macaques (Macaca mulatta) at the Tulane National Primate Research Center and in a large collection of simian acquired immunodeficiency syndrome – (SAIDS)-associated lymphomas. Quantification of RRV load was performed by real-time PCR using amplification primers specific for the RRV interleukin-6 homologue (RRV vIL-6). RRV infection was detected infrequently and at low levels in PBMC of randomly selected healthy animals. Examination of longitudinally collected PBMC from 22 SIV-infected animals throughout progression to SAIDS revealed similarly low RRV loads that sometimes increased with advancing disease. RRV infection was detected more frequently in the peripheral blood of SIV-infected animals than in healthy animals. Examination of SAIDS-associated lymphomas showed that RRV is rare within the tumor mass, likely representing infection in an occasional tumor-infiltrating cell or contaminating blood. The results indicate that RRV infection in PBMC is not predictive of, and is apparently not required for, development of lymphoma or hyperplastic lymphadenopathy in SIV-infected animals at TNPRC.