Controlling concentration of bioactive components in cat's claw based products with a hybrid separation process
- Resource Type
- Authors
- Bence Kószó; Kinga Komka; Anna Lisa Oelbermann; Thomas Gamse; Tibor Keve; Edit Székely; M. Maier; Sergio Sanz; Alba Calvo; Eckhard Weidner; Dániel Dévényi
- Source
- The Journal of Supercritical Fluids. 125:50-55
- Subject
- chemistry.chemical_classification
Chromatography
biology
010405 organic chemistry
DPPH
General Chemical Engineering
Alkaloid
Extraction (chemistry)
Supercritical fluid extraction
Fractionation
Condensed Matter Physics
biology.organism_classification
01 natural sciences
0104 chemical sciences
010404 medicinal & biomolecular chemistry
chemistry.chemical_compound
chemistry
Uncaria tomentosa
Tannin
Organic chemistry
Oxindole
Physical and Theoretical Chemistry
- Language
- ISSN
- 0896-8446
Supercritical fluid extraction (SFE) with and without entrainer combined with organic solvent extraction was used for fractionation of bioactive natural products as alkaloids and antioxidants from cat's claw ( Uncaria tomentosa (Willd.) DC.) bark. Extracts obtained from this plant have a number of beneficial effects, including anti-inflammatory or immune stimulator. The aim of this work was to obtain extracts of enriched and limited alkaloid concentrations, good antioxidants and tannin agents. The results demonstrated that a hybrid process involving SFE is a selective method to obtain an oxindole alkaloid rich fraction (186.0 ± 3.2 mg total pentacyclic oxindole alkaloids/g extract) and a product enriched in tannins (23.8 ± 1.9% tannin content in extract) and antioxidants (IC 50 = 5.88 ± 0.54 μg/ml extract).