Effect of rotation on turbine blade internal cooling is an important factor in gas turbine cooling systems. In order to minimize the impact from the Coriolis force, cooling structures with less rotation-dependent cooling effectiveness are needed. This study presents an impingement design in a two pass channel to reduce impact of rotational forces on non-uniform heat transfer behavior inside these complex channels. A Transient Liquid Crystal(TLC) method was employed to obtain local heat transfer coefficient measurements in a rotating environment. The channel Reynolds number based on the channel diameter ranges from 25,000 to 100,000. The rotation number ranges from 0 to 0.14. A series of computational simulations with the SST model were also utilized to understand the flow field behavior that impacts the heat transfer distributions on the walls. A 1-D correlation of zone averaged Nusselt number distribution was derived from the measurements. Results show that rotation reduces the heat transfer on both sides of the impingement, which is due to the Coriolis force and the pressure redistribution. The local change in the present study is about 25%. Rotation significantly enhances the heat transfer near the closed end because of the centrifugal force and the ‘pumping’ effect. Within the parameters of this test, the magnitude of enhancement is 25% to 75%. Compared to U-bended two pass channel, impingement channel has advantages in the upstream channel and the end region, but performance is not beneficial on the leading side of the downstream channel.