Hyperpolarization-activated cation currents in medium-size dorsal root ganglion cells are involved in overactive bladder syndrome in rats
- Resource Type
- Authors
- Kun Zhang; Liping Yao; Guojun Wu; Jianlin Yuan; Jun-Ling Xing; Fei Yan; Chao Tan; Fei Liu; Weijun Qin
- Source
- BMC Urology, Vol 20, Iss 1, Pp 1-12 (2020)
BMC Urology
- Subject
- medicine.medical_specialty
Urology
030232 urology & nephrology
Action Potentials
Stimulus (physiology)
urologic and male genital diseases
I h
lcsh:RC870-923
ZD7288
03 medical and health sciences
0302 clinical medicine
Dorsal root ganglion
Internal medicine
Cations
Ganglia, Spinal
medicine
Animals
Patch clamp
Urinary bladder
medicine.diagnostic_test
business.industry
Urinary Bladder, Overactive
Overactive bladder
General Medicine
Syndrome
Hyperpolarization (biology)
medicine.disease
lcsh:Diseases of the genitourinary system. Urology
Overactive bladder syndrome
female genital diseases and pregnancy complications
Rats
medicine.anatomical_structure
Endocrinology
Hyperpolarization
Reproductive Medicine
030220 oncology & carcinogenesis
Urodynamic testing
Female
business
Research Article
- Language
- English
- ISSN
- 1471-2490
Background To investigate the functions of the hyperpolarization-activated cation currents in medium-size dorsal root ganglion cells in a rat model of overactive bladder syndrome. Methods Rats with OAB were screened using a urodynamic testing device. The whole-cell patch clamp technique was used to investigate changes in excitability and hyperpolarization-activated cation current (Ih) of medium-size cells in the L6 dorsal root ganglia (DRG) of the OAB rats. Intrathecal injection of the specific Ih inhibitor ZD7288 was used to investigate changes of voiding function and Ih of medium-size cells in the L6 DRG. Results The urinary bladder weight of the OAB rats was significantly increased (p p Ih current density, which was blocked by ZD7288. Conclusions The Ih current density significantly increased in medium-size cells of the L6 DRG in the OAB model. A decrease of the Ih current was able to significantly improve the voiding function of the OAB rats, in addition to lowering their urinary bladder weight. Our finding suggested that the observed increase of Ih current in the medium-size DRG neurons might play an important role in the pathological processes of OAB.