Purpose: To evaluate the effectiveness of a one-day and three-day train-the-trainer HIV/AIDS program delivered to homeopathy and ayurveda practitioners and educators. Introduction: The purpose of the training was to assess (a) the change in knowledge of and attitudes regarding HIV/AIDS at three and six months following the training, and (b) the number of secondary trainees trained by the primary trainees. Methods: Participants included 202 physicians of homeopathy and ayurveda in northern India. A total of 96 physicians of homeopathy and ayurveda were trained as primary trainees in the one-day program, and 106 were trained in the three-day program. Knowledge of and attitudes toward HIV/AIDS were measured by a modified 21-item questionnaire. The one-day training program covered epidemiology of the disease, transmission, and clinical presentation with a case-study approach. The three-day training added the social, ethical, and legal impact of being HIV-infected along with health counselor and participant role plays. Results: Findings revealed that both the one-day and three-day primary trainees significantly increased their HIV/AIDS knowledge and attitude scores from pretest to immediate posttest with ongoing improvement in both groups over the three- and six-month period. A total of 26% of primary trainees in the one-day program trained secondary trainees, and 36% of primary trainees in the three-day program trained secondary trainees. Conclusions: A train-the-trainer approach can improve the knowledge and attitude scores of ayurveda and homeopathy practitioners and educators as well as increase the number of secondary trainees. Increasing the number of trained providers to reinforce HIV prevention messages has policy implications for the Indian System of Medicine, which serves nearly 2.5 million HIV-infected patients.