PDF file - 53KB, Suitable amplification of genomic loci from three microdissected ACF samples was accomplished using the Sequenom standard PCR protocol. Microdissected ACF DNA achieved the equivalent amplification levels obtained using 1 ng of input DNA. The loci amplified is a 462 nt region of RRBP1 using the following primers - F (exon) - 5'-AGATGGCGAAAACTCACCACC-3' and R (intron) - 5'- GCCTTTTTGCCCTGAGTAGT-3'. The primers span an intron-exon border to restrict amplification to the genomic DNA template.