Detection and Characterization of Xanthomonas vasicola pv. vasculorum (Cobb 1894) comb. nov. Causing Bacterial Leaf Streak of Corn in the United States
- Resource Type
- Authors
- Mary Ortiz-Castro; John Rascoe; Kevin A. Korus; E. DuCharme; Emily K Luna; Tamra A. Jackson-Ziems; J. Ibarra Caballero; Kirk Broders; Jan E. Leach; Jillian M. Lang; Terra Hartman
- Source
- Phytopathology®. 107:1312-1321
- Subject
- 0301 basic medicine
Whole genome sequencing
Veterinary medicine
Xanthomonas
Phylogenetic tree
Xanthomonas vasicola
food and beverages
Virulence
Gene Expression Regulation, Bacterial
Plant Science
Biology
biology.organism_classification
Zea mays
United States
Housekeeping gene
03 medical and health sciences
030104 developmental biology
Bacterial Proteins
Phylogenetics
Botany
Agronomy and Crop Science
Phylogeny
Bacterial leaf streak
Plant Diseases
- Language
- ISSN
- 1943-7684
0031-949X
Bacterial leaf streak of corn (Zea mays) recently reached epidemic levels in three corn-growing states, and has been detected in another six states in the central United States. Xanthomonas vasicola was identified as the causal agent of this disease. A multilocus sequence alignment of six housekeeping genes and comparison of average nucleotide identity from draft genome sequence were used to confirm phylogenetic relationships and classification of this bacteria relative to other X. vasicola strains. X. vasicola isolates from Nebraska and South Africa were highly virulent on corn and sugarcane and less virulent on sorghum but caused water-soaking symptoms that are typical of X. vasicola infection on the leaves of all three hosts. Based on host range and phylogenetic comparison, we propose the taxonomic designation of this organism to X. vasicola pv. vasculorum ( Cobb 1894 ) comb. nov. Polymerase chain reaction-based diagnostic assays were developed that distinguish X. vasicola pv. vasculorum and X. vasicola pv. holcicola from each other and from other Xanthomonas spp.