Additional file 1: Supplementary Figure 1. Immunofluorescence staining of human brain tissue with astrocyte and neuron markers. a. Immunofluorescence staining of human brain tissue of normal subjects showed co-localization of LILRB2 and TREM2 with microglial marker IBA1 (top row). The bottom row showed no co-localization of LILRB2 and TREM2 with astrocyte marker GFAP. Scale bar = 20 μm. b-c. Binding kinetics profiles between oAβ and LILRB2 or TREM2. In the association stage, protein A sensor-captured LILRB2-Fc (b) or TREM2-Fc (c) protein was incubated with oAβ at indicated concentrations The amount of oAβ bound onto the sensors was presented as wavelength shift in nanometers (nm). The red dotted vertical line marks the transit from the association stage to the dissociation stage, where the sensors were dipped into kinetics buffer without oAβ allowing free dissociation. The binding kinetics parameters were calculated using a 1:1 binding model with global fitting.