DETECTION OF RICKETTSIA TSUTSUGAMUSHI FROM VECTOR TROMBICULID MITE BY DNA AMPLIFICATION USING POLYMERASE CHAIN REACTION TECHNIQUE
- Resource Type
- Authors
- Tiansheng Chen; Hong Ni; Jiacan Li; Xiaoying Zheng
- Source
- Insect Science. 1:322-332
- Subject
- biology
Scrub typhus
bacterial infections and mycoses
Dna amplification
medicine.disease
biology.organism_classification
Virology
Molecular biology
General Biochemistry, Genetics and Molecular Biology
law.invention
Rickettsia
law
Insect Science
medicine
Mite
Vector (molecular biology)
Antigen Gene
Agronomy and Crop Science
Gene
Ecology, Evolution, Behavior and Systematics
Polymerase chain reaction
- Language
- ISSN
- 1744-7917
1672-9609
With reference to the Sta 58 major antigen gene of Rickettsia tsutsugamushi (Karp strain), we had designed and synthesized a pair of DNA primers; and a kilobase fragment of Sta 58 is amplified for using in polymerase chain reaction (PCR) with the primers. This is the first report on detection of R. tsutsugamushi in trombiculids by using PCR technique. The experimental results indicated that the time for which R. tsutsugamushi could exist in the adult of Leptotromhidium deliense when inoculated into its abdomenal cavity was 360 days at least and R. tsutsugamushi could be transovarially transmitted to the offsprings for 4 generations; and the time for which R. tsutsugamushi could exist in L. deliense after biting the infective mouse was 270 days at least and R. tsutsugamushi could be transmitted transovarially to the offsprings for 2 generations. The results also showed that the PCK technique was highly specific and sensitive for detection of R. tsutsugamushi; and the amplification of gene outside body to detect the rickettsiae in the body of trombiculid and mouse can be used as a new method for investigation of scrub typhus epidemiology.