Additional file 1: of A common antigenic motif recognized by naturally occurring human VH5–51/VL4–1 anti-tau antibodies with distinct functionalities
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- Apetri, Adrian; Crespo, Rosa; Juraszek, Jarek; Pascual, Gabriel; Janson, Roosmarijn; Xueyong Zhu; Zhang, Heng; Keogh, Elissa; Trevin Holland; Wadia, Jay; Verveen, Hanneke; Berdien Siregar; Mrosek, Michael; Taggenbrock, Renske; Jeroenvan Ameijde; Inganäs, Hanna; Winsen, Margot Van; Koldijk, Martin; Zuijdgeest, David; Borgers, Marianne; Dockx, Koen; Stoop, Esther; Wenli Yu; Linden, Els Brinkman-Van Der; Ummenthum, Kimberley; Kolen, Kristof Van; Mercken, Marc; Steinbacher, Stefan; Marco, Donata De; Hoozemans, Jeroen; Wilson, Ian; Koudstaal, Wouter; Goudsmit, Jaap
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- mental disorders
urologic and male genital diseases
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Figure S1. Peptide epitope mapping. Figure S2. Reactivity of CBTAU-27.1 and CBTAU-28.1 to PHF-tau. Figure S3. Affinity of CBTAU-27.1, CBTAU-28.1 and their affinity-matured mutants for their cognate tau peptides. Figure S4. Affinity-improved antibodies dmCBTAU-27.1 and dmCBTAU-28.1 retain both the nature and specificity of the interactions of the parental antibodies with tau. Figure S5. Detection of immunoreactivity in various tauopathies by CBTAU-27.1 and CBTAU-28.1 and affinity-improved variants. Figure S6. Set-up of an in vitro rtau aggregation assay. Figure S7. Complete dataset for in vitro tau aggregation in the absence or presence of CBTAU-27.1 and dmCBTAU-27.1. Figure S8. Complete dataset for in vitro tau aggregation in the absence or presence of CBTAU-28.1 and dmCBTAU-28.1. Figure S9. Complete dataset for in vitro tau aggregation in the absence or presence of Fab-CBTAU-27.1 and Fab-dmCBTAU-27.1. Figure S10. Complete dataset for in vitro tau aggregation in the absence or presence of Fab-CBTAU-28.1 and Fab-dmCBTAU-28.1. Figure S11. In vitro tau aggregation in the presence of dmCBTAU-27.1 added at different time points. Figure S12. Assessment of CBTAU-27.1 binding to rtau and PHFs by SEC-MALS. Figure S13. Macroscopic image of rtau fibrils generated in the absence and presence of CBTAU-28.1. Figure S14. Tau aggregates are internalized by BV-2 cells and localize in cellular acidic organelles. Table S1. Names and sequences of tau peptides used in this study. The first 10 peptides listed were used as baits in the BSelex method. Table S2. Data collection and refinement statistics for CBTAU-27.1 Fab and CBTAU-28.1 Fab. Table S3. Data collection and refinement statistics for dmCBTAU-27.1 - A8119 and dmCBTAU-28.1 - A7731 complexes. (DOCX 30952 kb)