Total knee arthroplasty (TKA) is a common treatment for osteoarthritis. Recently, there has been interest in the anatomical differences between male and female knees and possible effects on outcome. The purpose of this study is to analyze whether gender influenced on clinical outcome, radiographic findings, revision rate, or complications of TKAs performed with unisex components. Six hundred eighty-one patients (28% male and 72% female) treated with TKA were evaluated. Similar patient age, body mass index, International Knee Society (IKS) knee score, range of motion, and stage of osteoarthritis were noted in both groups pre-operative. IKS function score and activity level were higher in the male group. At a mean of 2.3 years post-operative, both groups demonstrated similar increases in IKS scores. Function scores and activity levels remained higher in the male group. No differences in radiologic findings or revision rates were noted. Slightly higher infection rates were noted in the male group.