New method for the estimation of platelet ascorbic acid
- Resource Type
- Authors
- Harry Lander; J. V. Lloyd; P. S. Davis
- Source
- Journal of Clinical Pathology. 22:453-457
- Subject
- Blood Platelets
Resolution (mass spectrometry)
Iron
Ascorbic Acid
Chloride
Pathology and Forensic Medicine
Ferrous
Chlorides
Spectrophotometry
Methods
medicine
Platelet
Chromatography
medicine.diagnostic_test
Triazines
Chemistry
Elution
Articles
General Medicine
Ascorbic acid
Phenylhydrazines
Biochemistry
Ferric
Chromatography, Thin Layer
Oxidation-Reduction
medicine.drug
- Language
- ISSN
- 0021-9746
Present techniques for the estimation of platelet ascorbic acid allow interference by other substances in the sample. A new and more specific method of analysis is presented. The proposed method owes its increased specificity to resolution of the extract by thin-layer chromatography. By this means ascorbic acid is separated from other reducing substances present. The separated ascorbic acid is eluted from the thin layer and estimated by a new and very sensitive procedure: ascorbic acid is made to react with ferric chloride and the ferrous ions so formed are estimated spectrophotometrically by the coloured derivative which they form with tripyridyl-Striazine. Results obtained with normal blood platelets were consistently lower than simultaneous determinations by the dinitrophenylhydrazine (DNPH) method.