N-myc gene is amplified in alveolar rhabdomyosarcomas (RMS) but not in embryonal RMS
- Resource Type
- Authors
- J. Heighway; H. R. Gattamaneni; Shant Kumar; Henry B Marsden; P. Dias; Patricia Kumar
- Source
- International journal of cancer. 45(4)
- Subject
- Male
Cancer Research
Pathology
medicine.medical_specialty
Biology
Gene duplication
Rhabdomyosarcoma
medicine
Humans
Child
Southern blot
Gene Amplification
Infant
Histology
DNA, Neoplasm
Oncogenes
medicine.disease
Prognosis
Exact test
Blotting, Southern
Oncology
Child, Preschool
Alveolar rhabdomyosarcoma
Female
Embryonal rhabdomyosarcoma
N-Myc
- Language
- ISSN
- 0020-7136
DNA from 13 (6 alveolar and 7 embryonal) childhood rhabdomyosarcomas (RMS) was examined to determine the incidence and prognostic relevance of N- and c-myc genes. Southern analysis showed 5- to 20-fold amplification of N-myc gene in 4 of 6 alveolar but in none of 7 embryonal RMS (p less than 0.04; Fisher's exact test). The number of children who died with multiple- and single-copy N-myc gene was 4/4 and 5/9 respectively (p greater than 0.05; Chi-squared test). There was no statistically significant correlation between N-myc amplification and age, gender, site, stage or survival time. There was no amplification or gross rearrangement of c-myc in any of the 13 RMS.