Estimation of Airborne Rat-Derived Antigens by ELISA
- Resource Type
- Authors
- M. Rackham; Audrey V. Thompson; G. E. Davies
- Source
- Journal of Immunoassay. 4:113-126
- Subject
- Male
Pharmacology
Chromatography
Chemistry
Coefficient of variation
Immunology
Enzyme-Linked Immunosorbent Assay
Rats, Inbred Strains
Double antibody sandwich
Allergens
Rats
Immunoenzyme Techniques
Absorbance
Linear relationship
Animals laboratory
Antigen
Animals, Laboratory
Immunoenzyme techniques
Hypersensitivity
Animals
Humans
Animal Husbandry
Antigens
Skin
Filter material
- Language
- ISSN
- 0197-1522
Double antibody sandwich ELISA procedures have been devised to estimate the concentrations of airborne rat urinary and epidermal antigens. Both assays were capable of detecting at least 3 ng/ml of antigen protein. Unknown concentrations were estimated in terms of the standard, laboratory-prepared material, as calculated from the linear relationship between absorbance and log concentration. The coefficient of variation of a single assay was 12%, the methods have been applied to extracts of the filter material from static and personal samplers in an animal room.