The saturation of the best quality land, especially in the littoral regions ; the expansive tourist traffic and its aggressive forms ; the penetration of an increasing number of tourists in protected areas, especially in the strict natural reserves ; destructive forms of tourist construction, which completely occupy and devour the natural space and destroy the landscape cause systematic environmental damage. Such condition requires changes in behaviour towards the natural and cultural environment and the new paradigm of sustainable tourism development. This paper decomposes into their key elements definitions of both sustainable development and sustainable tourism development. The paper critically reviews the ecological-spatial sustainability, in particular the consistency and coherence of its indicators. For the purpose of the survey on the concrete and measurable impact assessment of sustainability, the ecological-spatial sustainability is broken down into components and indicators which form the basis of the questionnaire. The survey of tourist actors in the observed area was carried out in response to the NUTS 3 region. Results of statistical tests show that if we consider three indicators that can be influenced by stakeholders (waste production-environmental pollution, use of renewable energy sources, and conservation of the ecosystem), we can see significant statistical differences in the influence. Furthermore, the tests show that there is a difference in approach of stakeholders on how to improve these indicators dependant on their activities, number of employees, salary level and the affiliation with formal associations (clusters), which can affect the increase of ecological-spatial sustainability through selected indicators.