Epilepsy-induced changes in signaling systems of human and rat postsynaptic densities
- Resource Type
- Authors
- Eckart D. Gundelfinger; Ursula Wyneken; Fernando Orrego; Sergio Villanueva; Rodrigo Sandoval; Dagoberto Soto; Juan-Jose Marengo
- Source
- EPILEPSIA
Artículos CONICYT
CONICYT Chile
instacron:CONICYT
- Subject
- Adult
Male
Kainic acid
Adolescent
Down-Regulation
Tropomyosin receptor kinase B
Biology
Receptors, N-Methyl-D-Aspartate
Rats, Sprague-Dawley
Epilepsy
chemistry.chemical_compound
Neurotrophic factors
Postsynaptic potential
medicine
Animals
Humans
Receptor, trkB
Child
Cerebral Cortex
Neocortex
Kainic Acid
Infant
medicine.disease
Rats
Up-Regulation
medicine.anatomical_structure
nervous system
Neurology
chemistry
Child, Preschool
Synapses
Female
Neurology (clinical)
Epileptic seizure
medicine.symptom
Postsynaptic density
Neuroscience
Signal Transduction
- Language
- ISSN
- 0013-9580
Summary: Purpose: To study seizure-induced changes in signaling proteins present in postsynaptic densities (PSDs) isolated from human epileptic neocortex and from rat cortex in which seizures were induced by injection of kainic acid. Methods: We performed Western blot analysis of signaling proteins in PSDs isolated from cortical tissue. Results: Seizures induce a strong upregulation of TrkB, the receptor for brain-derived neurotrophic factor (BDNF), whereas components of the N-methyl-d-aspartate (NMDA)-receptor complex are downregulated in both human and rat PSDs. Conclusions: These data show that long-term changes in PSD composition occur as a consequence of epileptic seizure activity.