Foci of cyclin A2 interact with actin and RhoA in mitosis
- Resource Type
- Authors
- Mariya Georgieva; Shaereh Mashayekhan; Marion Peter; Abdelhalim Loukil; Jean-Marie Blanchard; Fanny Izard; Andrea Parmeggiani
- Source
- Scientific Reports
Scientific Reports, Nature Publishing Group, 2016, 6, pp.27215. ⟨10.1038/srep27215⟩
- Subject
- 0301 basic medicine
RHOA
[PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph]
Cyclin A
Cyclin B
Mitosis
[SDV.CAN]Life Sciences [q-bio]/Cancer
Polo-like kinase
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
Article
03 medical and health sciences
Cell Line, Tumor
Autophagy
Humans
[NLIN.NLIN-CG]Nonlinear Sciences [physics]/Cellular Automata and Lattice Gases [nlin.CG]
Biochemical switches in the cell cycle
[NLIN.NLIN-AO]Nonlinear Sciences [physics]/Adaptation and Self-Organizing Systems [nlin.AO]
Multidisciplinary
biology
[PHYS.PHYS.PHYS-SOC-PH]Physics [physics]/Physics [physics]/Physics and Society [physics.soc-ph]
G1/S transition
[SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN]
Actins
Cell biology
Enzyme Activation
030104 developmental biology
MCF-7 Cells
biology.protein
Cancer research
rhoA GTP-Binding Protein
Cyclin A2
- Language
- English
- ISSN
- 2045-2322
Cyclin A2 is a key player in the regulation of the cell cycle. Its degradation in mid-mitosis depends primarily on the ubiquitin-proteasome system (UPS), while autophagy also contributes. However, a fraction of cyclin A2 persists beyond metaphase. In this work, we focus on cyclin A2-rich foci detected in mitosis by high resolution imaging and analyse their movements. We demonstrate that cyclin A2 interacts with actin and RhoA during mitosis and that cyclin A2 depletion induces a dramatic decrease in active RhoA in mitosis. Our data suggest cyclin A2 participation in RhoA activation in late mitosis.