Simultaneous Intracellular Detection of Plasmonic and Non-Plasmonic Nanoparticles Using Dark-Field Hyperspectral Microscopy
- Resource Type
- Authors
- Melike Saricam; Anna Danilushkina; Rawil Fakhrullin; Mustafa Culha; Farida Akhatova; Gamze Kuku
- Source
- Bulletin of the Chemical Society of Japan. 91:1640-1645
- Subject
- Plasmonic nanoparticles
Chemistry
technology, industry, and agriculture
Hyperspectral imaging
Nanoparticle
Nanotechnology
02 engineering and technology
General Chemistry
respiratory system
010402 general chemistry
021001 nanoscience & nanotechnology
01 natural sciences
Dark field microscopy
respiratory tract diseases
0104 chemical sciences
Dark-field microscopy
Lung epithelium
Microscopy
Hyperspectral microimaging
0210 nano-technology
Spectral mapping
Intracellular
Plasmon
- Language
- ISSN
- 1348-0634
0009-2673
Herein we report the detection and differentiation of plasmonic and non-plasmonic nanoparticles simultaneously administered to A549 lung epithelium cells using dark-field microscopy and hyperspectral imaging. Reflectance spectra-based hyperspectral mapping and image analysis allows for the effective quasi-quantitative identification of nanomaterials in cultured human cells. © 2018 The Chemical Society of Japan. The work is performed according to the Russian Government Program of Competitive Growth of Kazan Federal University and funded by Russian presidential grant MD-6655.2018.4 and by the subsidy allocated to Kazan Federal University for the state assignment in the sphere of scientific activities (6.7743.2017/6.7).